| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti B220/CD45R A647 (Clone RA3-6B2) | BD Biosciences | #557683 |
| Anti CCR6 PerCP-Cy5.5 (Clone 29-2L17) | BioLegend | #129809 |
| Anti CD103 biotin (Clone 2E7) | Fischer Scientific eBioscience | #15239669 |
| Anti CD103 PE (Clone 2E7) | ThermoFisher eBioscience | #12-1031-82 |
| Anti CD11b PerCP-Cy5.5 (Clone M1/70) | BD Biosciences | #561114 |
| Anti CD11c PECy7 (Clone N418) | ThermoFisher eBioscience | #25-01114-82 |
| Anti CD169 FITC (Clone MOMA-1) | AbD Serotec | #MCA947F |
| Anti CD19 FITC (Clone 1D3) | BD Biosciences | #553785 |
| Anti CD19 PE (Clone 1D3) | BD Biosciences | #553786 |
| Anti CD19 A700 (Clone 6D5) | Fischer Scientific eBioscience | #115528 |
| Anti CD19 APC-Cy7 (Clone 6D5) | BioLegend | #115529 |
| Anti CD197 (CCR7) Purified (Clone 4B12) | BioLegend | #120101 |
| Anti CD197 (CCR7) Biotin (Clone 4B12) | ThermoFisher eBioscience | #13-1971-80 |
| Anti CD24 Pacific Blue (Clone M1/69) | BioLegend | #101820 |
| Ani CD26 BV786 (Clone H194-112) | BD Biosciences | #740868 |
| Anti-CD3 complex eF450 (Clone 17A2) | ThermoFisher eBioscience | #48-0032-82 |
| Anti CD3ε A700 (Clone eBio500A2) | Fischer Scientific eBioscience | #15217989 |
| Anti CD3ε PE (Clone 145-2C11) | BD Biosciences | #553064 |
| Anti CD4 APC-H7 (Clone GK1.5) | BD Biosciences | #560246 |
| Anti CD40 PE (Clone 3/23) | BD Biosciences | #553791 |
| Anti CD44 PE-Cy7 (Clone IM7) | ThermoFisher eBioscience | #25-0441-82 |
| Anti CD45.2 BUV395 (Clone 104) | BD Biosciences | #564616 |
| Anti CD69 FITC (Clone H1.2F3) | ThermoFisher eBioscience | #11-0691-82 |
| Anti CD80 APC (Clone 16-10A1) | BD Biosciences | #560016 |
| Anti CD86 PerCP/Cy5.5 (Clone GL-1) | BioLegend | #105028 |
| Anti CD8α BV711 (Clone 53-6.7) | BD Biosciences | #563046 |
| Anti CD8α APC (Clone 53-6.7) | BD Biosciences | #553035 |
| Anti CD8α Pacific Blue (Clone 53-6.7) | BD Biosciences | #558106 |
| Anti dsRED, dsRFP Purified (Rabbit Polyclonal IgG) | Rockland | #600-401-379 |
| Anti Fluorescein/Oregon Green A488 (Polyclonal) | ThermoFisher | #A11090 |
| Anti-Goat IgG A594 (Polyclonal Donkey Fab) | Jackson Immunoresearch | #705-587-003 |
| Anti GzmB APC (Clone GB11) | ThermoFisher | #GRB05 |
| Anti I-A/I-E (MHC-II) A700 (Clone M5/114.15.2) | ThermoFisher eBioscience | #56-5321-82 |
| Anti IL-12(p40/p70) APC (Clone C15.6) | BD Biosciences | #554480 |
| Anti IFNγ A700 (Clone XMG1.2) | BD Biosciences | #557998 |
| Anti IFNγ Biotin (Clone XMG1.2) | BD Biosciences | #554410 |
| Anti IFNγ Purified (Clone XMG1.2) | BioXCell | #BE0055 |
| Anti Ly49H biotin (Clone 3D10) | Fischer Scientific eBioscience | #15219899 |
| Anti MCMV IE1/m123 Purified (Clone IE1.01) | Capri/MEDRI | #IE1.01 |
| Anti mouse IgG2a A633 (Polyclonal Goat) | ThermoFisher | #A-21136 |
| Anti NK1.1 A700 (Clone PK136) | BD Biosciences | #560515 |
| Anti NK1.1 PE (Clone PK136) | BD Biosciences | #557391 |
| Anti NK1.1 PE-Cy7 (Clone PK136) | BD Biosciences | #552878 |
| Anti NK1.1 Purified (Clone PK136) | Purified in house | N/A |
| Anti NKp46/CD335 PE (Clone 29A1.4) | Fischer Scientific eBioscience | #15228749 |
| Anti NKp46/CD335 Purified (Polyclonal Goat IgG) | RnD Systems | #AF2225 |
| Anti rabbit IgG A546 (Polyclonal Donkey) | ThermoFisher | #A10040 |
| Anti rat IgG A594 (Polyclonal Donkey F(ab')₂) | Jackson Immunoresearch | #712-586-153 |
| Anti Siglec-H FITC (Clone 551) | BioLegend | #129604 |
| Anti SIRPα (CD172a) APC-Cy7 (Clone P84) | BioLegend | #144018 |
| Anti TCRβ FITC (Clone H57-597) | BD Biosciences | #553171 |
| Anti TCRβ PerCP/Cy5.5 (Clone H57-597) | BioLegend | #109228 |
| Anti TCRγδ BV711 (Clone GL3) | BD Biosciences | #563994 |
| Anti XCR1 APC (Clone ZET) | BioLegend | #148206 |
| Avidin A488 | ThermoFisher | #A21370 |
| Streptavidin APC | BD Biosciences | #554067 |
| Streptavidin eFluor 450 | ThermoFisher eBiosciences | #48-4317-82 |
| Streptavidin PE | BD Biosciences | #554061 |
| Streptavidin PE-Cy7 | BD Biosciences | #557598 |
| Virus strains | ||
| MCMV smith strain | ATCC | ATCC #VR-1399 |
| Chemicals, peptides, and recombinant proteins | ||
| 7α,25-Dihydroxycholesterol | Sigma-Aldrich | #SML0541-5MG |
| AntigenFix | DiaPath | #P0016 |
| m45 peptide HGIRNASFI | ProImmune | N/A |
| Poly(I:C) High Molecular Weight | InvivoGen | #tlrl-pic |
| Critical commercial assays | ||
| NK Cell Isolation Kit, mouse | Miltenyi Biotec | #130-115-818 |
| CD8+ Dendritic Cell Isolation Kit, mouse | Miltenyi Biotec | #130-091-169 |
| Deposited data | ||
| microarray data GSE142402 | GEO database | https://www.ncbi.nlm.nih.gov/geo/ |
| Experimental models: mouse strains | ||
| Ccr7−/− (B6.129P2(C)-Ccr7tm1Rfor/J) | The Jackson Laboratory | JAX #006621 |
| Cd45.1+/+ (B6.SJL-PtprcaPepcb/BoyJ) | The Jackson Laboratory | JAX #002014 |
| Csf2rb−/− (B6.129S1-Csf2rbtm1Cgb/J) | The Jackson Laboratory | JAX #005940 |
| Il12eYFP (B6.129-Il12btm1.1Lky/J) | The Jackson Laboratory | JAX #006412 |
| Il15rako/ko (Il15ratm1Ama) | The Jackson Laboratory | JAX #003723 |
| Il15raflox/flox (Il15ratm2.1Ama) | The Jackson Laboratory | JAX #022365 |
| KarmaCre (B6-Gpr141btm2Ciphe) | Mattiuz et al., 2018 | N/A |
| KarmatdTomato-hDtr (Gp141btm1Ciphe) | Alexandre et al., 2016 | N/A |
| Nkp46Cre (Ncr1tm1.1(iCre)Viv) | Narni-Mancinelli et al., 2011 | N/A |
| Rosa26lox−stop−lox−DTA (B6.129P2-Gt(ROSA)26Sortm1(DTA)Lky/J) | The Jackson Laboratory | JAX #009669 |
| Rosa26lox−stop−lox−tdRFP (Gt(ROSA)26Sortm1Hjf) | Luche et al., 2007 | N/A |
| Xcl1mTfp1-fl (Xcl1tm1Ciphe) | This paper | N/A |
| Xcr1−/− (B6.129P2-Xcr1tm1Dgen) | The Jackson Laboratory | JAX #005791 |
| Xcr1Cre (B6-Xcr1tm1Ciphe) | Mattiuz et al., 2018 | N/A |
| Oligonucleotides | ||
|
Csf2 forward GGCCTTGGAAGCATGTAGAGG |
This paper | N/A |
|
Csf2 reverse GGAGAACTCGTTAGAGACGACTT |
This paper | N/A |
|
Hprt forward TCAGTCAACGGGGGACATAAA |
This paper | N/A |
|
Hprt reverse GGGGCTGTACTGCTTAACCAG |
This paper | N/A |
|
Ifng forward CAACAGCAAGGCGAAAAAGG |
This paper | N/A |
|
Ifng reverse CCTGTGGGTTGTTGACCTCAA |
This paper | N/A |
|
Xcl1 forward AGAAGCCAAATGGGTGAAAGC |
This paper | N/A |
|
Xcl1 reverse TCAGCCATGTTCTTTCTGGTACTG |
This paper | N/A |
| Software and algorithms | ||
| BD FACSDiva™ Software v8.0 | BD Biosciences | https://www.bdbiosciences.com/en-us/instruments/research-instruments/research-software/flow-cytometry-acquisition/facsdiva-software |
| FlowJo v10.4 | Tree Star | https://www.flowjo.com |
| GraphPad Prism 7 | GraphPad | https://www.graphpad.com/scientific-software/prism |
| Icy Bioimage analysis | Icy Bioimage analysis | http://icy.bioimageanalysis.org |
| ImageJ | NIH | https://imagej.nih.gov/ij/download.html |
| Zen | Zeiss | https://www.zeiss.fr/microscopie/produits/microscope-software/zen-lite/zen-2-lite-download.html |
| Imaris | Oxford Instruments | https://imaris.oxinst.com |
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