Figure 5.

EPS11 directly targets collagen I.A, EPS11 protected target protein (collagen I) against temperature-dependent degeneration in Huh7.5 cells. B, the target affinity (equilibrium dissociation constant [KD] value) of EPS11 binding to collagen I was detected by SPR. C, collagen I was pulled down using FITC beads. FITC beads were incubated with cell lysates of Huh7.5 cells, and after washing steps, the complexes were finally obtained for Western blotting analysis. M, marker; 1, total cell lysates; 2, flow through (the bead-unbound proteins); 3, FITC pull-down (the bead-bound proteins). EPS11, exopolysaccharide 11; SPR, surface plasmon resonance.