TABLE 1.
BrdU incorporation in salivary gland cells expressing DREF polypeptides
Expected genotype of larvaea | No. of salivary glands (pairs) | No. of cells
|
No. of BrdU+ cells/salivary gland pair (mean ± SD) | |
---|---|---|---|---|
BrdU+ | BrdU− | |||
P[Sg-Gal4]; + ; + | 12 | 1,172 | 308 | 97 ± 16 |
w or Y + + | ||||
P[Sg-Gal4]; P[UAS-DREF1–155]; + | 12 | 168 | 1,088 | 12 ± 4 |
w or Y + + | ||||
P[Sg-Gal4]; P[UAS-DREF1–155]; P[UAS-DREF1–709]TM6 or P[Sg-Gal4]; P[UAS-DREF1–155]; Pr | 12 | 618 | 816 | 52 ± 14 |
w or Y ++w or Y ++ |
P[Sg-Gal4]/Binsinscy females were mated to transgenic males carrying P[UAS-DREF]. Half of their progeny, therefore, carried the Binsinscy chromosome, while the other half did not. Larvae without Binsinscy were monitored.