Skip to main content
. 2021 Sep 10;118(37):e2100624118. doi: 10.1073/pnas.2100624118

Fig. 2.

Fig. 2.

CREBA bidirectionally regulates LTM in DAL neurons. (A) Acute RNAi down-regulation of CREBA (–CREBA) in DAL impaired 1-d memory after 10×S (Left). LTM was similarly blocked by CXM feeding (Center Left). Memory was unaffected at 18 °C after 10×S (Center Right) and at 30 °C after 10×M (Right). (B) Overexpressing crebA (+CREBA) in DAL neurons at 30 °C enhanced 1-d memory after 3×S (Left) and persisted at least 4 d (Center Left). Memory was unaffected at 18 °C after 3×S (Center Right) and at 30 °C after 3×M (Right). (C) Similarly, overexpressing crebA in DAL neurons enhanced 1-d memory after 1× (Left), persisting at least 4 d (Center). Memory was unaffected at 18 °C after 1× (Right). (D) Overexpressing crebA in DAL neurons enhanced 1-d memory after 10×S. In all figures, temperature control schedules are indicated (Top). Flies were raised at 18 °C and then transferred to 30 °C to remove GAL4 inhibition by GAL80ts for 3 d before training. Bars represent mean ± SE, n = 8/bar. *P < 0.05; **P < 0.01; ***P < 0.001.