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. 2021 Sep 10;118(37):e2100624118. doi: 10.1073/pnas.2100624118

Fig. 3.

Fig. 3.

CREBA bidirectionally regulates CaMKII and per transcription in DAL neurons. (A) The CaMKII promoter was activated in DAL neurons 24 h after 10×S but not after 10×M, as reported by de novo KAEDE fluorescent protein synthesis (Left) (refer to Fig. 1 for details). Elevated de novo KAEDE synthesis was suppressed following RNAi down-regulation of CREBA (–CREBA, Right). (B) The per promoter was similarly activated in DAL neurons 24 h after 10×S but not 10×M (Left). This activity was also abolished by RNAi down-regulation of CREBA (Right). (C and D) By contrast, levels of CaMKII (Left) and per (Right) gene activation were elevated by crebA overexpression (+CREBA) after 3×S and 1× but not after 3×M. Flies were raised at 18 °C and then transferred to 30 °C to remove GAL4 inhibition by GAL80ts for 3 d before training. Refer to SI Appendix, Figs. S3 and S4 for representative images. Bars represent mean ± SE, n ≥ 4/bar. *P < 0.05; **P < 0.01; ***P < 0.001.