Fig. 4.

Increased DAL neuron membrane excitability activates genes and enhances LTM. (A) NaChBac overexpression (+NaChBac) increased DAL neurons excitability and elevated 1-d memory after 3×S (Left), persisting for at least 4 d (Center Left). Memory was unaffected at 18 °C after 3×S (Center Right). Memory was similarly unaffected after 3×M (Right). (B) NaChBac overexpression also elevated 1-d memory after 1× (Left), persisting for at least 4 d (Center). Memory was unaffected at 18 °C after 1× (Right). (C) NaChBac overexpression in DAL neurons had no effect on 1-d memory after 10×S. (D and E) crebA (Top), CaMKII (Middle), and per (Bottom) activity in DAL neurons as reflected by de novo KAEDE synthesis (see Fig. 1) was elevated by NaChBac overexpression after 3×S and 1×. Refer to SI Appendix, Fig. S5 for representative images. Bars represent mean ± SE, n ≥ 6/bar. *P < 0.05; ***P < 0.001.