Fig. 5.

Generation of escape mutants of human bNAbs DC2.M108 and DC2.M357. (A) Neutralization curves of DC2.M16, DC2.M108, and DC2.M357 bNAbs against WT and mutant rVSV-CHIKV. Data shown are from two independent experiments performed in triplicate wells (points represent mean ± SD). IC₅₀ values were determined by fitting a nonlinear regression, and 95% confidence interval is shown. Escape mutations for DC2.M108 and DC2.M357 mapped to the CHIKV p62-E1 heterodimer structure (B) (Protein Data Bank [PDB] ID: 3N40) and the E1/E2 heterohexamer (C) (PDB ID: 3J2W). C-α spheres indicate positions of escape mutations for DC2.M108 (cyan), DC2.M357 (magenta), as well as CHK-265 escape mutations against RRV (silver). E1 is colored gray.