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. 2021 Sep 10;118(37):e2107665118. doi: 10.1073/pnas.2107665118

Fig. 1.

Fig. 1.

Generation and characterization of SOFIA founder pigs and offspring. (A) Cartoon of the β cell–specific INS-SNAP expression vector with 1.3-kb upstream regions, exon 1 to 3 and intron 1 of the porcine INS gene, in frame SNAP-tag sequence and a polyadenylation (pA) cassette of the bovine growth hormone gene, linked to a floxed neomycin resistance cassette. The sites of primers used for genotyping are indicated. (B) Southern blot analysis for the evaluation of integration pattern. Integration patterns of 11 F0 founder pigs and nine F1 offspring of founder 1,817 are shown. Genomic DNA was digested with the “null cutter” restriction endonuclease enzyme NdeI. *, the band representing the endogenous INS promoter; **, neo/δ-neo transgene integration site with two integrants where neo cassette was deleted only in one INS-SNAP integrant; and ***, δ-neo transgene integration site. (C) Immunofluorescence labeling against SNAP-tag and insulin in transgenic offspring in the SOFIA neo/δ-neo F2 generation. (Scale bar, 20 µm.) Detail shows the magnified boxed region. (Scale bar, 5 µm.)