Fig. 6.
E-cad dynamics in complex with β-cat are diffusive. (A) Normalized time decays of the E-cad ABC construct at two temperatures (indicated). Solid and dashed lines are fits with an exponential function and with the Smoluchowski equation, respectively. (B) Relaxation times of the E-cad ABC construct as a function of temperature. The solid line is the dependence without activation energy (SI Appendix). The red area indicates the 90% confidence interval of this fit. The dashed line is an Arrhenius fit. (Inset) DA distance distributions for E-cad bound (black) and free (gray) from smFRET-based CG model. (C) Comparison of the diffusion coefficient of E-cad free (gray circle) and bound to β-cat (white circles). Shaded areas represent the effect of different roughness values (σ in kBT). Black solid and dashed lines are fits with the Zwanzig expressions (60) for periodic- (σ = 4.3 ± 0.1) and Gaussian- (σ = 2.9 ± 0.1) modulated roughness, respectively. (D) Scheme of E-cad on the surface of β-cat. Parts of E-cad can partially detach, thus giving access to regulatory enzymes. (E) Same as D but illustrating the spring-like properties of the E-cad/β-cat complex.