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Coexpression of DagA and EXB3 in C. glutamicum SP002.
A. Coexpression system of pHCP‐CgR‐DagA‐EXB3. pCG1 ori and p15A ori indicate the origin of replication for C. glutamicum and E. coli, respectively.
B. SDS‐PAGE analysis of the culture supernatant by fed‐batch cultivation. Each lane represents protein size markers (M) and culture times (0–48 h). EXB3 (◀) and DagA (◁) are indicated as arrowheads.
