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. 2021 Jun 17;14(5):1918–1930. doi: 10.1111/1751-7915.13834

Fig. 5.

Fig. 5

Analysis of purified Med‐ORF10 by electrophoresis and gel shift assay

A. Med‐ORF10 pre‐treated with different denaturing agents and analysed by SDS‐PAGE (left panel). M: Protein marker; lane 1: Med‐ORF10 without addition of loading buffer; lane 2: Med‐ORF10 with loading buffer; lane 3: Med‐ORF10 mixed with loading buffer and 2% SDS; lane 4: Med‐ORF10 mixed with loading buffer and 2% SDS and 250 mM DTT; and lane 5: Med‐ORF10 mixed with loading buffer and 250 mM DTT.

B. Med‐ORF10 pre‐treated with different denaturing agents and then analysed by Native‐PAGE (right panel). M: Protein marker; lane 1: Med‐ORF10 without addition of loading buffer; lane 2: Med‐ORF10 mixed with loading buffer containing 100 mM DTT; lanes 3‐4: Med‐ORF10 mixed with loading buffer containing 2% SDS and 250 mM DTT; and lane 5: Med‐ORF10 mixed with loading buffer containing 2% SDS.

C. Location of a 182‐bp promoter region of med‐ORF12 in med gene cluster for MED biosynthesis.

D. EMSA including biotin‐labelled 182‐bp promoter DNA incubated with different amounts of Med‐ORF10 (left panel) and positive control (right panel).