TABLE 2.
Plasmids used in this study
| Plasmid | Vector | Description | Reference or source |
|---|---|---|---|
| pBGM18 | pRS316 | URA3-CEN ADH2-lacZ reporter plasmid (codon 109 of ADH2 fused to lacZ) | This lab |
| pLGADH2-lacZ | pLG669Z | 2μm-URA3 ADH2-lacZ reporter plasmid (codon 23 of ADH2 fused to lacZ) | This lab |
| pHDY10 | pLG669Z | 2μm-URA3 UAS1 reporter plasmid | This lab |
| YIp23ADH2-lacZ | YIp5 | Integrating TRP1 ADH2-lacZ reporter plasmid | 21 |
| pKD63 | pRS316 | URA3-CEN plasmid with REG1 | This study |
| pKD89 | pRS316 | URA3-CEN plasmid with HA-REG1 | This study |
| pKD93 | pRS316 | pKD89 with the Δ1 deletion | This study |
| pKD94 | pRS316 | pKD89 with the Δ2 deletion | This study |
| pKD95 | pRS316 | pKD89 with the Δ3 deletion | This study |
| pKD96 | pRS316 | pKD89 with the Δ4 deletion | This study |
| pKD92 | pRS316 | pKD89 with the Δ5 deletion | This study |
| pKD97 | pRS316 | pKD89 with the Δ6 deletion | This study |
| pKD98 | pRS316 | pKD89 with the Δ7 deletion | This study |
| pKD104 | pRS316 | pKD89 with the Δ8 deletion | This study |
| pKD111 | pRS316 | pKD89 with the Δ9 deletion | This study |
| pKD112 | pRS316 | pKD89 with the Δ10 deletion | This study |
| pKD114 | pRS316 | pKD89 with the I466M F468A substitutions | This study |
| pKD115 | pRS316 | pKD95 with the deleted region restored | This study |
| pKD109 | pRS316 | pKD63 with REG1 fused in frame at codon 1002 to GFP | This study |
| pKD110 | pRS316 | pKD89 with HA-REG1 fused in frame at codon 1002 to GFP | This study |
| pRSM306 | pRS306 | pRS306 with the 2μm origin from YEp24 | T. Davis |
| pKD123 | pRSM306 | URA3 2-hybrid lexA-REG1 expression plasmid | This study |
| pKD125 | pRSM306 | URA3 2-hybrid lexA-REG1 plasmid with the Δ3 deletion | This study |
| pKD126 | pRSM306 | URA3 2-hybrid lexA-REG1 plasmid with the Δ8 deletion | This study |
| pKD127 | pRSM306 | URA3 2-hybrid lexA-REG1 plasmid with the I466M F468A substitutions | This study |
| pGAD-GLC7 | pGAD | LEU2 2-hybrid GAD-GLC7 plasmid | 57 |
| pTT49 | pGAD | LEU2 2-hybrid GAD-ORC1 plasmid | R. Sternglanz |