TABLE 3.

Oligonucleotide primers used in plasmid constructions

Oligonucleotide primer	Sequence (5′→3′)
62-A	ACCACCTCCTGAAAGAGAAC
62-B	AGCGTAGTCTGGCACGTCATATGGGTACATTTTTGGATTTTTCTTATCTCGTCTTCG
62-C	ATGTACCCATATGACGTGCCAGACTACGCTTCAACAAATCTAGCAAATTACTTCGCCG
62-D	CAATATATTCATCAAGAAGGCCC
89-1-3′	GTGCCAGACTACGCTAGATCTTGGAGAACATGGGC
89-1-5′	GCCCATGTTCTCCAAGATCTAGCGTAGTCTGGCAC
89-2-5′-N	CTTCTCCCTTGTCACCAGATCTCCATTCATGTGACAG
89-2-3′-N	GCCAGAAAGACATGTTAGATCTAATTCAAATGGTGGCGG
89-3-3′	CGAACAGTAGCGTTAGATCTGAAGAACACGGCGG
89-3-5′	CCGCCGTGTTCTTCAGATCTAACGCTACTGTTCG
89-4-3′	CCATTGCTAGCCATTCAGAGATCTTCATCAGATAGCG
89-4-5′	CGCTATCTGATGAAGATCTCTGAATGGCTAGCAATGG
89-5-3′	GAAAAAAGATCTAGTGATGTTGCCATAGAGGG
89-5-5′	ATCACTAGATCTTTTTTCCTTGGATTCTACCGC
89-6-3′	CACCAGCAAACAGATCTTAGAAGAAAGAATTTTGAAGTCAAC
89-6-5′-N	ATACGCTAGATCTAACCAGCTGACTGTTAGCGGGTAATGGTC
89-(455-475)-3′	CCTCAAACCCAAGTGAAAGATCTTGTATGGCACTACGATATCC
89-(455-475)-5′	GGATATCGTAGTGCCATACAAGATCTTTCACTTGGGTTTGAGG
89-(349-554)-3′	CCGTGAGATCTATCATCAGAATGTGATGAAGATGATGATTGTG
89-(349-554)-5′	AACAGTGGATCCAGTTTGAAGAGTCAACACTCTGAC
pRS-5′	GCAACTGTTGGGAAGGGCGATCGGTGCGGG
pRS-3′	CCATGATTACGCCAAGCTCGGAATTAACCC
89-I466M, F468A-3′	CCTACTAAAAATAGACATATGCATGCTAATGACAGGGTGG
89-I466M, F468A-5′	CCACCCTGTCATTAGCATGCATATGTCTATTTTTAGTAGG
XhoI-GFP-3′	CACTATCTCGAGAATTGGAGCTCGGTACCAG
AatII-GFP-5′	TCTAGGACGTCCGCAGGCGCTGGAGCCGGTG