TABLE 4.

Glc7 proteins defective in repressing SUC2 expression fully repress ADH2 expression

Strain	Relevant genotype	β-Galactosi-dase activitya (Miller units)		Invertase activityb (nmol)
		R	DR	R	DR
SB90	GLC7	3	450	3	200
SB241(HA)	glc7-131	6	320	54	170

^aADH2 expression was assayed as β-galactosidase activity expressed by the ADH2 reporter plasmid pLGADH2-lacZ. These values are the means for at least two independent transformants assayed in duplicate and had a maximum standard deviation of 30%. Repressed cells (R) had 5% glucose and derepressed cells (DR) had 2% glycerol, 2% lactate, 2% ethanol, and 0.1% glucose as the carbon sources, respectively. 

^bSUC2 expression was assayed as invertase activity in nanomoles of sucrose hydrolyzed per min per unit of optical density at 600 nm of culture used. Each assay was performed in duplicate and had an average range of 30%. R had 5% glucose and DR had 0.1% glucose as the carbon source, respectively.