TABLE 5.
Deletion of snf1 or bcy1 suppresses constitutive ADH2 expression
| Strain | Relevant genotype | β-Galactosidase activitya (Miller units)
|
|
|---|---|---|---|
| R | DR | ||
| KDY80 | BCY1 REG1 SNF1 | 9.5 | 1,140 |
| KDY88 | BCY1 REG1 snf1 | 6.0 | 6.0 |
| KDY90 | bcy1 REG1 SNF1 | 4.8 | 9.0 |
| KDY82 | BCY1 reg1 SNF1 | 220 | 2,740 |
| KDY89 | BCY1 reg1 snf1 | 4.9 | 4.8 |
| KDY92 | bcy1 reg1 SNF1 | 12 | 22 |
a
ADH2 expression was assayed as β-galactosidase activity expressed from the integrated reporter YIp23ADH2-lacZ. The values for KDY80 and KDY82 are means of three independent assays performed in duplicate. All other values are means for three independent disruptants derived from either KDY80 or KDY82 and assayed in duplicate. Each measurement had a standard deviation of less than 20%. Repressed cells (R) had 5% glucose and derepressed cells (DR) had 3% ethanol as the carbon source, respectively.