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. 2021 Sep 18;7:253. doi: 10.1038/s41420-021-00644-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Schematic showing 2000-bp of the promoter region of porcine TGF-β1. Transcription start site (TSS) was considered to be +1. MRE1, miRNA response element 1 for miR-2337. MRE2, miRNA response element 2 for miR-2337. B, C Identification of the core promoter region. Four deleted constructs p227, p366, p675, and p1328 were generated (B), and transfected into GCs. Luciferase assays were measured (C). D Schematic annotation of the report vector of the TGF-β1 promoter containing the MRE motif. E, F The effect of miR-2337 on the promoter activity of the TGF-β1 gene. Report vectors of the TGF-β1 promoter containing MRE1 (E) or MRE2 (F) motif were co-treated with miR-2337 mimics, and luciferase activity was measured. G, H The effect of miR-2337 on TGF-β1 expression in GCs with the genotype AA/AA/AA. GCs were isolated from sows with the genotype AA/AA/AA and transfected with miR-2337 mimics, TGF-β1 mRNA (G) and protein (H) level was detected. Data are represented as means ± S.E.M. (n = 3). *p < 0.05.