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. 2021 Sep 18;7:251. doi: 10.1038/s41420-021-00648-0

Fig. 4. TMZ ameliorates DEX-induced muscle atrophy in mice.

Fig. 4

Mice were intraperitoneally injected with 0.9% saline (Ctrl), DEX (25 mg/kg), TMZ (5 mg/kg), or DEX (25 mg/kg) +TMZ (5 mg/kg) for 10 days. A Body weight of mice. n = 8 per group. B Exercise capacity testing. a. Running distance. b. Running time. n = 8 per group. C Grip strength test. n = 8 per group. D Comparison of representative samples of dissected skeletal muscle including gastrocnemius (Gast), tibialis anterior muscle (TA), soleus muscle (Sol), and quadriceps (Quad). n = 4 per group. E Representative H&E staining of myofiber cross-section of Gast. Scale bar = 100 μm. A microscope with a 10× objective was used to capture the images. n = 4 per group. F Protein levels of Atrogin-1 and MuRF1 in Gast muscle. n = 4 per group. G Protein levels of p85α PI3K, p-AKT, total AKT, p-FOXO3a, and total FOXO3a in Gast muscle. n = 4 per group. *P < 0.05 vs. Ctrl. **P < 0.01 vs. Ctrl. #P < 0.05 vs. DEX. ##P < 0.01 vs. DEX. DEX dexamethasone, TMZ trimetazidine, Ctrl control.