Table 1.

Summarized antigen and adjuvant concentrations for co-lyophilized formulations. In addition to antigen and CoVaccine HT (CoV) at the specified concentrations, each formulation also contained 9.5% (w/v) trehalose and 10 mM ammonium acetate at pH 7. CoVaccine HT concentration refers to the concentration of the immunostimulant SFASE.

Study	Group ID	EBOV-GP (ug/mL)	SUDV-GP (ug/mL)	MARV-GP (ug/mL)	CoVaccine HT (mg/mL)
Study A: Adjuvant Dose Response Study in Mice (Dose Volume = 0.1 mL)	E1	10	---	---	0
	E10	100	---	---	0
	E1+CoV0.3	10	---	---	3
	E10+CoV0.3	100	---	---	3
	E1+CoV0.5	10	---	---	5
	E10+CoV0.5	100	---	---	5
	E1+CoV0.7	10	---	---	7
	E10+CoV0.7	100	---	---	7
	E1+CoV1	10	---	---	10
	E10+CoV1	100	---	---	10
	CoV1	---	---	---	10
Study B: Antigen Dose Response Study in Mice (Dose Volume = 0.1 mL)	E0.1+CoV0.3	1	---	---	3
	E1+CoV0.3	10	---	---	3
	S0.1+CoV0.3	---	1	---	3
	S1+CoV0.3	---	10	---	3
	M0.1+CoV0.3	---	---	1	3
	M1+CoV0.3	---	---	10	3
	E0.1+S0.1+M0.1+CoV0.3	1	1	1	3
	E1+S1+M1+CoV0.3	10	10	10	3
Study C: NHP Immunogenicity Study (Dose Volume = 0.5 mL)	E25+CoV5	50	---	---	10
	E25+S25+M10+CoV5	50	50	20	10
	E25+M10+CoV5	50	---	20	10
	E25+S25+M5+CoV5	50	50	10	10
	CoV5	---	---	---	10