TABLE 1.

Sequences of oligonucleotide primers used in the amplification of the pbp1A gene

Primera	Sequence (5′ to 3′)	Position in pbp1A geneb	Product length (bp) after amplification with downstream primer 1A-2
1A-R1	AAGAACACTGGTTATGTA	2662–2679	224
1A-R2	AGCATGCATTATGCAAAC	2317–2334	569
1A-1	ACAAATGTAGACCAAGAAGCTCAA	1843–1866	1,043
1A-2	TACGAATTCTCCATTTCTGTAGAG	2863–2886

^aPrimers 1A-1 and 1A-2 are specific for pneumococci. Primers 1A-R1 and 1A-R2 specifically amplify pneumococcal isolates for which the penicillin MICs are ≥0.25 and ≥1 μg/ml, respectively. 

^bAccording to the sequence data of Smith and Klugman (22).