TABLE 3.

Pneumococcal isolates showing discrepant pbp1A and pbp2B results compared with their MIC data^a

Isolate no.	Penicillin MIC (μg/ml)	pbp2B		pbp1A
		PCR (resistance) productb	Nucleotide sequence data compared to strain R6c	PCR (resistance) product		Nucleotide sequence data compared to strain R6c
				1A-R1	1A-R2
29	0.125	−	Identical to R6	+	−	Mutations
36	0.25	−	Identical to R6	+	−	Mutations
89	0.125	−	Identical to R6	+	−	Mutations
129	0.5	−	Mutations	−	−	Mutations
139	0.12	−	Mutations	−	−	Mutations
143	0.25	−	Identical to R6	−	−	Mutations

^aIsolates were sequenced through the penicillin-binding domain encoding regions. 

^bThe pbp2B PCR assay was done as previously described by du Plessis et al. (6). 

^cR6 is a penicillin-susceptible strain.