Table 1.
Primer | Sequence | Temperature |
---|---|---|
Adora2a |
Fwd: AGCAACCTGCAGAACGTCACAAAC Rev: TGGCAATAGCCAAGAGGCTGAAGA |
60 |
Arc |
Fwd: GGAGGGAGGTCTTCTACCGTC Rev: CCCCCACACCTACAGAGACA |
57.5 |
C3 |
Fwd: GACGCCACTATGTCCATCCT Rev: CCAGCAGTTCCAGGTCCTTTG |
57.5 |
cFos |
Fwd: CTCTGGGAAGCCAAGGTC Rev: CGAAGGGAACGGAATAAG |
55 |
Egr1 |
Fwd: GAGGAGTTATCCCAGCCAA Rev: GGCAGAGGAAGACGATGAAG |
57.5 |
GAPDH |
Fwd: GTGTTTCCTCGTCCCGTAGA Rev: ATTCCGTTCACACCGACCTT |
55, 57.5, 60 |
IL3 |
Fwd: CCTGGGACTCCAAGCTTCAA Rev: GACAATAGAGCTGCAATTCAACGT |
57.5 |
Il6 |
Fwd: ACGGCCTTCCCTACTTCACA Rev: CATTTCCACGATTTCCCAGA |
57.5 |
TNF-a |
Fwd: GGTGCCTATGTCTCAGCCTCTT Rev: GCCATAGAACTGATGAGAGGGAG |
60 |