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. 2021 Sep 18;17:308. doi: 10.1186/s12917-021-03013-6

Table 2.

The primer sequences used in this study

Primers Primer sequences(5´-3´) Length of product/bp Reference
ORF2-F ACGTCATCTCCGTTGGAACC 226
ORF2-R TGGAGCCAAGTGTTTGTGGT
PCR1-F ATTATGGATGCTCCTCATCGTG 553 (Wen, et al. [14])
PCR1-R CATCTTCTCCGCAACTTCAGTC
PCR2-F GACTGAAGTTGCGGAGAAGATG 789
PCR2-R CGGCACGAAAGAAGTTTGGATT
PCR3-F CCCACATGCGAGGGCGTTTACC 895
PCR3-R CGAGGCCGCTTCATCATCCACT