Table 3.
Comparisons of the biochemical properties of various mesothermal microbial β-amylases
| Microorganism | GenBank accession no. | Specific activity (U/mg) | Optimum temperature (°C) | Optimum pH | Host | Plasmid | Reference |
|---|---|---|---|---|---|---|---|
| B. aryabhattai | This study | 3798.9 | 50 | 6.0 | Bacillus subtilis TEB1030 | pBE-S | This study |
| B. cereus | P36924.2 | 2182 | 40 | 7.0 | NR | NR | [25] |
| B. flexus | RIV10038.1 | 3092 | 50 | 7.0–8.0 | E.coli BL21(DE3) | pET24a(+) | [36, 39] |
| P. polymyxa | 3VOC_A | 1500 | 45 | 7.5 | B. polymyxa No. 26 − 1(WT) | no | [35] |
| B. firmus | P96513.1 | NR | 55–60 | NR | E.coli HB101 | pUC18 | [38] |
| B. circulans | P06547.1 | 0.77 μm/mg/min | 50 | 7.0 | E.coli Rosetta2 | pET-21a(þ) | [18] |
| Halobacillus sp. LY9 | NR | NR | 60 | 8.0 | Halobacillus sp. LY9(WT) | no | [19] |
| B. megaterium | WP_013081506.1 | NR | 50 | 7.5 | B. megaterium DSM319 | pDAMY1 | [40] |
NR: not reported; WT: wild type strain