Multiphoton intravital microscopy was used to image neutrophil/S. aureus interactions in vivo for 10 min at 24 h post-infection.
(A) Representative image taken from time-lapse videos showing neutrophils at the infection site from WT, ΔarlRS, and ΔmgrA skin infections.
(B) Quantification of neutrophil track displacement length in the x-y position in S. aureus skin infections.
(C and D) Quantification of the mean displacement of neutrophils per minute (velocity).
Data are shown as mean ± SEM. n = 5–7. *p < 0.05. See also Videos S1 and S2.