Multiphoton intravital microscopy was used to image neutrophil/S. aureus interactions in vivo at 24 h post-infection.
(A) Representative intravital image showing a three-dimensional stitched image viewed from the x-z plane (side view) showing neutrophil localization at the infection site.
(B and C) Image analysis quantification of total neutrophil spots at the infection site.
(D and E) Total S. aureus surface volume at the infection site.
(F and G) S.-aureus-positive neutrophils.
(H and I) Percentage of S. aureus volume that was infiltrated by neutrophils.
Data are shown as mean ± SEM. n = 6–11. *p < 0.05; **p < 0.01. See also Videos S3 and S4.