Skip to main content
. Author manuscript; available in PMC: 2021 Sep 19.
Published in final edited form as: Cell Rep. 2021 Jul 27;36(4):109462. doi: 10.1016/j.celrep.2021.109462

KEY RESOURCES TABLE.

REAGENT or RESOURCE SOURCE IDENTIFIER
Bacterial and virus strains
S. aureus USA300 CA-MRSA, ermS (= LAC) Boles et al., 2010 AH1263
S. aureus LAC Δagr::tetM Kiedrowski et al., 2011 AH1292
S. aureus LAC Δsak::tetM This Paper AH4990
S. aureus LAC nuc::LtrB Kiedrowski et al., 2011 AH1680
S. aureus LAC chs:: φNΣ This Paper AH4960
S. aureus LAC lukA:: φNΣ This Paper AH4963
S. aureus LAC lukS:: φNΣspc This Paper AH4987
S. aureus LAC Δebh Crosby et al., 2016b AH3150
S. aureus LAC ΔarlRS Walker et al., 2013 AH1975
S. aureus LAC ΔarlRS φ11::LL29tet arlRS Kwiecinski et al., 2019 AH3244
S. aureus LAC ΔarlRS Δagr::tetM This Paper AH3288
S. aureus LAC ΔarlRS Δsak::tetM This Paper AH5599
S. aureus LAC ΔarlRS::tetM Crosby et al., 2016b AH3520
S. aureus LAC ΔarlRS::tetM Δebh This Paper AH3151
S. aureus LAC ΔarlRS::tetM sraP::φNΣ This Paper AH3817
S. aureus LAC ΔarlRS::tetM sraP::φNΣ Δebh This Paper AH3818
S. aureus LAC ΔmgrA::tetM Crosby et al., 2016b AH3455
S. aureus LAC ΔmgrA::tetM φ11::LL29erm mgrA Crosby et al., 2016b AH3485
S. aureus LAC ΔmgrA::tetM Δebh Crosby et al., 2016b AH3481
S. aureus LAC ΔmgrA::tetM sraP::φNΣ Crosby et al., 2016b AH3811
S. aureus LAC ΔmgrA::tetM sraP::φNΣ Δebh Crosby et al., 2016b AH3798
S. aureus LAC ΔmgrA Crosby et al., 2016b AH3375
S. aureus LAC ΔmgrA Δagr::tetM This Paper AH4986
S. aureus ST5 MSSA (= 502A) Parker et al., 2014 AH3610
S. aureus 502A ΔarlRS::tetM Crosby et al., 2016b AH3624
S. aureus 502A ΔmgrA::tetM Crosby et al., 2016b AH3625
S. aureus USA400 MRSA (= MW2) Baba et al., 2002 AH843
S. aureus MW2 ΔarlRS::tetM Crosby et al., 2016b AH3060
S. aureus MW2 ΔmgrA::tetM Crosby et al., 2016b AH3456
S. aureus USA100 MRSA (= N315) Kuroda et al., 2001 AH2398
S. aureus N315 ΔarlRS::tetM Crosby et al., 2016b AH3082
S. aureus N315 ΔmgrA::tetM Crosby et al., 2016b AH3473
S. aureus USA200 MSSA (= MN8) Schlievert and Blomster, 1983 AH2413
S. aureus MN8 ΔarlRS::tetM Crosby et al., 2016b AH3063
S. aureus MN8 ΔmgrA::tetM Crosby et al., 2016b AH3480
L. lactis: surrogate host for ClfA expression O’Brien et al., 2002 MG1363
Biological samples
Whole blood, human volunteers N/A
Plasma, human volunteers N/A
Serum, human Innovative Research Cat# ISER10ML
Chemicals, peptides, and recombinant proteins
Serum albumin, human Millipore Sigma Cat# 12667
Fibrinogen, human Millipore Sigma Cat# F3879
Polymorphprep Accurate Chemical Cat# AN1114683
Syto 9 stain Thermo Fisher Scientific Cat# S34854
Propidium Iodide Thermo Fisher Scientific Cat# L10316
Carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) BioLegend Cat# 423801
Streptokinase Millipore Sigma Cat# S0577
Trypsin Millipore Sigma Cat# T4799
DNase I Millipore Sigma Cat# DN25
Phorbol 12-myristate 13-acetate (PMA) Millipore Sigma Cat# 524400
N-Formyl-Met-Leu-Phe (fMLP) Millipore Sigma Cat# F3506
HNP-1 (human α-defensin 1) AnaSpec Cat# AS-60743
FRET oligonucleotide substrate Integrated DNA Technologies (Kavanaugh et al., 2019) Custom synthesis
Sodium hypochlorite ACROS Organics Cat# 419550250
Collagen I, rat BD Biosciences Cat# 354236
Critical commercial assays
iTaq Universal SYBR Green Supermix Bio-Rad Cat# 1725121
LDH Cytotoxicity Detection Kit Millipore Sigma Cat# 11644793001
Experimental models: cell lines
Dermal fibroblasts, murine C57BL/6J female mice, isolated according to Khan and Gasser (2016) N/A
Experimental models: organisms/strains
Mouse: C57BL/6J Jackson Laboratories RRID:IMSR_JAX:000664
Mouse: CatchupIVM-red: C57BL/6-Ly6g(tm2621(Cre-tdTomato)Arte) Hasenberg et al., 2015 N/A
Oligonucleotides
See Table S1
Recombinant DNA
pJB38; mutation generation vector, CmR / AmpR Wörmann et al., 2011 N/A
pCM28; S. aureus - E. coli shuttle vector, CmR / AmpR Pang et al., 2010 N/A
pCM29; sGFP expression vector, CmR / AmpR Pang et al., 2010 N/A
pHC66; mgrA complementing vector (pCM28::mgrA), CmR / AmpR Crosby et al., 2016b N/A
pJK09; sak deletion vector (pJB38 sak::tetM), CmR / AmpR This Paper N/A
pKS80; L. lactis expression vector, ErmR O’Brien et al., 2002 N/A
pKS80::clfA; vector for expression of ClfA in L. lactis, ErmR O’Brien et al., 2002 N/A
Software and algorithms
FIJI - ImageJ Schindelin et al., 2012 https://imagej.net/software/fiji/
CFX Manager, v. 3.1 Bio-Rad https://www.bio-rad.com/en-us/sku/1845000-cfx-manager-software?ID=1845000
Imaris, v. 9.5.1 Oxford Instruments https://imaris.oxinst.com/versions/9-5
Prism, v.7 GraphPad Software https://www.graphpad.com/scientific-software/prism
Other
Angiogenesis μ-slide, tissue culture treated Ibidi Cat# 81506
8 well μ-slide, tissue culture treated Ibidi Cat# 80826
CFX96 Touch Real-Time PCR System Bio-Rad N/A
BZ-X710 microscope Keyence N/A
Eclipse TE2000-E microscope Nikon N/A
Leica TCS SP8 2-photon microscope Leica Microsystems N/A
InSight DeepSee laser Spectra-Physics N/A