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. 2021 Aug 14;49(16):9594–9605. doi: 10.1093/nar/gkab673

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Conversion efficiency on multi-genes of CRISPR-CDA-nCas9-UGI. (A) Conversion efficiency of double genes (secY and secE) editing. (B) Conversion efficiency of triple genes (secY, secE, and secG) editing. The conversion efficiency of the first and second passage for the double and triple genes was compared. The asterisks indicate significant editing based on a comparison between the experimental group and control group (**P <0.01, Student's t-test). Conversion efficiency increased with the multiple iterations for the (C) double and (D) triple genes. The direction of the arrow indicates the increase with the passage. Bars represent the average editing efficiency and error bars represent the S.D. of three independent biological replicates.