Figure 2.

risiRNAs were enriched in exosome mutants. (A) Schematics of the exosome complex and the subunits in C. elegans. (B) Images show seam cells of the respective L2 stage animals expressing GFP::NRDE-3. Numbers indicate the percentage of animals with nucleus-enriched NRDE-3 in seam cells (% N). The number of scored animals is indicated in parentheses. Scale bars, 5 μm. Schematics of the alleles are shown in Supplementary Figure S3. (C) Results from the deep sequencing of total small RNAs from L3 animals. The green dashed lines indicate risiRNAs. (D) Results from the deep sequencing of NRDE-3-associated siRNAs from L3 animals.