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. 2021 Aug 7;49(16):9194–9210. doi: 10.1093/nar/gkab662

Figure 4.

Figure 4.

risiRNAs induced an association of NRDE proteins with pre-rRNAs. (A) Schematic of the rDNA transcription unit and real-time PCR primers. The two thick red bars indicate the dsRNA segments targeting 18S rRNAs. (B, C) Expression levels of rRNAs and pre-rRNAs quantified by real-time PCR after exogenous RNAi targeting 18S rRNA. (D, E) Relative amount of NRDE protein-bound pre-rRNAs by immunoprecipitating NRDE proteins followed by quantitative real time PCR of the associated RNAs. lin-15b is a protein coding gene, which is used as a positive control for RIP experiments. (F) ChIP analysis of rDNA loci upon treatment of RNAi targeting the lin-15b gene or 18S rRNA. Trimethylation of H3K9 and H3K27 were measured. Data are presented as mean ± s.d.; n = 3; *P < 0.05, **P < 0.01, ***P < 0.001, n.s., not significant.