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. 2021 Aug 7;49(16):9194–9210. doi: 10.1093/nar/gkab662

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — risiRNAs induced a nucleolar accumulation of NRDE-2. (A) Schematic of the dsRNAs targeting different regions of rDNA. (B) (top panel) Images of C. elegans embryos expressing GFP::NRDE-2 (green) and mCherry::RPOA-2 (red) after animals were fed with indicated dsRNA clones. lin-15b is a protein coding gene, which is used as a negative control. Scale bars, 10 μm. (botton panel) The percentage of embryos showing the colocalization of NRDE-2 with RPOA-2 were quantified. (C) Images of C. elegans embryos expressing GFP::NRDE-2 (green) and mCherry::RPOA-2 (red) in indicated animals grown at 20 and 15°C respectively. For the nucleolar NRDE-2 foci in embryos at 15°C in disl-2 and dis-3 mutants, worms were cultured at 15°C from L3 stage to adult stage, then embryos were photographed. Numbers indicate the percentages of the animals with nucleolar-enriched NRDE-2 in embryos (%NCL). The number of scored animals is indicated in parentheses.