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MsPA reduces cytokine induction observed with model pro-inflammatory gapmer ASOs in BJAB cells. Sequence and design of ASOs and fold increase in CCl22 mRNA in BJAB cells as measured by qRT-PCR, following treatment with 1.6 mM ASO by free-uptake for 16 h. Blue letters indicate cEt, black DNA, red—2′-O-Me, subscript pink u = MsPA, s = PS, superscript m = 5-Me group on cytosine nucleobases.
