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. 2021 Aug 11;49(16):9211–9228. doi: 10.1093/nar/gkab672

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 10. — Transposition frequency of ISPpu9 in different genetic backgrounds. (A) Representation of plasmid pKN-ISPpu9Km, which contains the complete ISPpu9 insertion sequence and a kanamycin-resistant determinant inserted downstream of the tnp gene. Asr9 is represented in red, while Ssr9 is depicted in blue. (B and C) The pKN-ISPpu9Km suicide delivery plasmid, or its derivative lacking ssr9 (pKN-ISPpu9KmΔssr9), were transferred by conjugation into strains KT2440 or F1, as specified in Materials and Methods section; the number of transposition events per recipient is indicated. (D and E) Plasmids pSEVA225T (control for conjugation; D) and plasmid pUT-Mini-Tn5Km (control for transposition; E) were transferred to strains KT2440 and F1 using the same procedure. Values are the average of five independent assays.