Figure 6.

Hybridization of Asr9 to Ssr9. (A) Regions of sequence complementarity in Asr9 and Ssr9; alignment was performed using intaRNA software (79) at http://rna.informatik.uni-freiburg.de. (B) Increasing amounts of unlabelled Asr9 were mixed with a fixed amount of radioactively labeled Ssr9, and the complexes formed were resolved by non-denaturing gel electrophoresis. The presumed Ssr9/Asr9 complexed formed is indicated. (C) Binding of Hfq protein to Ssr9 and Asr9. Increasing amounts of purified Hfq (0–400 nM) were added to labeled Ssr9 or Asr9, and the complexes formed were resolved by non-denaturing gel electrophoresis. (D) Effect of Hfq on the formation of an Asr9/Ssr9 hybrid. Reaction mixtures were prepared as in (B) but in the absence or presence of purified Hfq protein (50 nM).