FIGURE 2.

RAB14 knockdown affects asymmetric division in mouse oocytes. (A) Real‐time quantitative PCR results of the Rab14 mRNA expression level in the Rab14 siRNA‐injected group and the control group. ***, significant difference (P < .001). (B) Western blot analysis for RAB14 expression in the Rab14 siRNA‐injected group and control group. Relative intensity of RAB14 and α‐tubulin was assessed by densitometry. **, significant difference (P < .01). (C) Diagram for the rescue approach by Myc‐Rab14 mRNA microinjection after RAB14 knock down. (D) DIC images of control oocytes, RAB14‐KD oocytes and rescue oocytes after 12 h culture. RAB14 knock down caused large polar bodies in mouse oocytes (black arrows). (E) Rate of polar body extrusion and large polar body extrusion after 12 h culture of the control group, Rab14 siRNA‐injected group and the rescue group. No significant difference for the polar body extrusion between these three groups (P > .1). Rate of large polar body extrusion after 12 h culture increased in the Rab14 siRNA‐injected group compared with the control group and rescue group. **, significant difference (P < .01)