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. 2021 Jul 29;54(9):e13104. doi: 10.1111/cpr.13104

FIGURE 3.

FIGURE 3

RAB14 knockdown impairs meiotic spindle positioning in mouse oocytes. (A) Representative images of spindle morphology (green) and chromosome alignment (blue) in the control and RAB14‐KD oocytes. No significant difference for the rate of bipolar spindle between these two groups (P > .1). Bar = 5 μm. (B) Representative images of the spindle positioning in the control and RAB14‐KD oocytes. Green, α‐tubulin; blue, DNA. Bar = 20 μm. The rate of spindle migration in the RAB14‐KD group decreased compared with the control group. **, significant difference (P < .01). (C) Representative images of the spindle positioning in RAB14‐KD and rescue oocytes. Green, α‐tubulin; blue, DNA. Bar = 20 μm. The rate of spindle migration increased in the rescue group compared with the RAB14‐KD group. **, significant difference (P < .01). (D) Calculation of spindle position to the cortex. The diameter of oocyte was defined as D, and the distance from spindle pole to oocyte cortex as L. The ratio of L/D increased in the Rab14 siRNA‐injected group compared with the control group, but decreased in the rescue group compared with the Rab14 siRNA‐injected group. ***, significant difference (P < .001)