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. 2021 Jul 29;54(9):e13104. doi: 10.1111/cpr.13104

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© 2021 The Authors. Cell Proliferation published by John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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RAB14 knockdown disrupts cytoplasmic actin assembly in mouse oocytes. (A) Representative images of actin filament distribution at the oocyte cortex and cytoplasm in the control group, RAB14‐KD group and rescue group. Red, F‐actin. Bar = 20 μm. (B) No significant difference was observed between control oocytes, Rab14 siRNA‐injected oocytes and rescue oocytes for the F‐actin fluorescent intensities at the cortex (P > .1); however, F‐actin fluorescent intensities in the cytoplasm were decreased in the Rab14 siRNA‐injected oocytes compared with the control and rescue oocytes. *, significant difference (P < .05). (C) Quantitative analysis of the relative intensity of ARP2, N‐WASP, ROCK1 and p‐cofilin in oocytes by Western blot. The results indicated that ROCK1 and p‐cofilin protein expression were significantly reduced in oocytes after RAB14 depletion. **, significant difference (P < .01); *, significant difference (P < .05). (D) Representative images of ROCK1 in the control and RAB14‐KD oocytes. ROCK1 fluorescent intensities decreased in the RAB14‐KD oocytes compared with the control oocytes. *, significant difference (P < .05). Bar = 20 μm