Skip to main content
. 2021 Sep 20;15:100276. doi: 10.1016/j.lanwpc.2021.100276

Figure 2.

Figure 2

Pseudovirus neutralization capacity of 50 plasma samples from BNT162b2 (Pfizer-BioNTech) vaccinees against SARS-CoV-2-Spike pseudoviruses bearing the Wuhan reference strain or the other 6 variant strains. (A) Amino acid changes (point mutations or deletions) relevant to the Wuhan reference strain of different SARS-CoV-2 variants, including B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma), B.1.617.1 (Kappa), B.1.617.2 (Delta), and B.1.429 (Epsilon). Orange filled boxes: changes at the receptor binding domain (RBD); Grey filled boxes: changes outside the RBD. (B) 50 plasma samples from BNT162b2 vaccinees collected 68 to 77 days (median 71 days) after the second dose of vaccination were tested at 1:20 dilutions for their neutralization potency against Wuhan reference strain (WT) or the other 6 variant strains. Statistical analysis was carried out to compare any two different pseudoviruses using Kruskal-Wallis tests followed by post hoc Dunn's multiple comparisons tests (*** P ≤ 0.001, **** P ≤ 0.0001). Only the comparison between the Wuhan reference strain (WT) and each of the other 6 variant strains plus that between the B.1.617.1 strain and the B.1.617.2 strain were shown in the figure. (C) Each individual plasma sample was arbitrarily defined as either ‘Strong neutralizer (red bar)’, or ‘Moderate neutralizer (pink bar)’, or ‘Weak neutralizer (grey bar)’ or ‘Non-neutralizer (black bar)’ if the percentage of neutralization at the 1:20 dilution is higher than 80%, between 50% to 80%, between 20 to 50%, and lower than 20%, respectively. The numbers indicated the combined percentage of ‘Weak neutralizers’ and ‘Non-neutralizers’ (0-50% neutralization) out of total 50 plasma samples for each different pseudovirus strains. (D) Comparison of EC50 values of neutralization between WT (black circles), and the B.1.617.2 strain (brown circles) pseudoviruses from 15 selected donor samples of either high responders against the B.1.617.2 strain (filled circles, n = 7) or low responders against the B.1.617.2 strain (empty circles, n = 8). Statistical analysis was carried out using paired two-tailed t-test ( **** P ≤ 0.0001).