Figure 1.

The primary cultured cells were observed under an inverted microscope. Endometrial cells were completely adherent within 24 hours and oviduct mucosa cells adherent after 48 hours. Both cells were polygonal, tadpole and spindle type in primary culture and early passage. The nucleus was centered and round, and the cells gathered and grew like islands. Most of the cells began to change in morphology after passage to the third generation, and gradually appeared long fusiform. By the fifth generation, most of the cells grew into fusiform, similar to fibrous cells. (A) Primary endometrial cells; (B) primary oviduct mucosal cells; (C) the third generation of endometrial cells; (D) the third generation of oviduct mucosal cells; (E) the fifth generation of endometrial cells; (F) the fifth generation of oviduct mucosal cells.