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. 2021 Sep 15;13:829–840. doi: 10.2147/IJWH.S328567

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© 2021 Wu et al.

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Immunofluorescence identification of epithelial marker keratin antibody was performed on endometrial cells and oviduct mucosa cells of the second generation. The results showed that 80–90% of the cytoplasm of endometrial cells and oviduct mucosa cells were stained with green fluorescence, showing positive cytokeratin antibodies. Microscopically, the cells were polygonal, spindle and spindle. Immunofluorescence identification of interstitial cell marker vimentin antibody was performed on endometrial cells and oviduct mucosa cells of the fifth generation. 80% of the cytoplasm of endometrial cells and oviduct mucosa cells were stained with red fluorescence under microscope. It showed positive cell vimentin antibody, and the cells were fusiform and polygonal under the microscope. (A1) The second generation of endometrial cells (DAPI). (A2) The second generation of endometrial cells (CK19). (A3) The second generation of endometrial cells (Merge). (B1) The second generation of oviduct mucosal cells (DAPI). (B2) The second generation of oviduct mucosal cells (CK19). (B3) The second generation of oviduct mucosal cells (Merge). (C1) The fifth generation of endometrial cells (DAPI). (C2) The fifth generation of endometrial cells (Vimentin). (C3) The fifth generation of endometrial cells (Merge). (D1) The fifth generation of oviduct mucosal cells (DAPI). (D2) The fifth generation of oviduct mucosal cells (Vimentin). (D3) The fifth generation of oviduct mucosal cells (Merge).