Figure 2.
Transient expression in N. benthamiana leaves, and confirmation of protein accumulation by confocal microscopy
(A) Selected leaves from a four-wee-old plant.
(B) Selected leaves agroinfiltrated into the abaxial side with a 1 mL needle-less syringe.
(C) Localization of the WT and the non-myristoylable (C4G2A) C4 versions fused to GFP. Zoom-out pictures: scale bar = 50 μm. Zoom-in pictures: scale bar = 10 μm. AF, autofluorescence.
(D) Two days post-agroinfiltration, CHX is infiltrated with a 1 mL needle-less syringe into the abaxial side of the same leaves previously agroinfiltrated.
(E) Two hours later, flg22 (or mock solution) is infiltrated with a 1 mL needle-less syringe into the abaxial side of the same leaves previously treated with CHX.