Stellaris hybridization buffer	Final concentration	Amount
Dextran Sulfate	100mg/mL	300 mg
Formamide	10%	300 μL
20× SSC	2×	300 μL
Nuclease free H2O	n/a	2.4 mL
Total	n/a	3 mL

This will make enough hybridization buffer for one sample. Excess hybridization buffer can be stored at −20°C but should only be freeze-thawed a maximum of twice.