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. 1999 Mar;37(3):638–648. doi: 10.1128/jcm.37.3.638-648.1999

FIG. 4.

FIG. 4

FIG. 4

FIG. 4

RFLP patterns of the pbp genes from penicillin-resistant isolates of S. pneumoniae from Bulgaria. (left panel) Fingerprints of pbp1a genes generated by HinfI; (middle panel) fingerprints of pbp2b genes generated by StyI; (right panel) fingerprints of pbp2x genes generated by MseI-DdeI. The outer lanes contain molecular size markers (pBR322 digested with HpaII). Lanes marked with R6 contain the penicillin-susceptible laboratory strain. Lanes marked with a black triangle contain the following additional control strains: lanes 3, Clev 2 (control for the serogroup 23F international clone A); lanes 7, IC 2 (control for another multiresistant internationally spread strain isolated in Iceland); lanes 18, Hun 524 (a serotype 19A multiresistant strain from Hungary); lanes 19, Cs 22 (a serotype 19A multiresistant strain from the Czech Republic). The rest of the lanes contained the following strains: lanes 1 and 2, Bul 89 and 22 (PFGE pattern A); lanes 4 to 6, Bul 72 and 78 and Sof 370 (PFGE pattern E; for the first two strains penicillin MICs were low and for the third they were high); lanes 8 to 10, Bul 45, 125, and 85 (PFGE pattern I); lanes 11 to 17, Bul 23, 29, 40, 91, and 115, Sof 289, and Bul 16 (all serogroup 19 and PFGE pattern K).