Fig. 1.

Schematic representation of SARS-CoV-2-DETECTR experimental workflow. In DETECTR, nucleic acid is amplified using reverse transcriptase-recombinase polymerase amplification (RT-RPA) or reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) technology followed by recognition of target dsDNA by Cas12a-crRNA complex, which triggers the Cas12a enzyme which possesses collateral/trans-cleavage activity and thus cleaves fluorescence tagged reporter ssDNA which produces visual readout analyzed on dip strip paper by lateral flow assay