FIGURE 2.

Response to VUAA1 of cells expressing bark beetle Orco genes. (A) Cells expressing the codon optimized I. typographus Orco gene (ItypOrco^HsCO; n = 6 biological replicates; n_total = 18) demonstrate increased maximal response and a somewhat lower EC₅₀ value as compared to cells expressing the wildtype Orco gene (ItypOrco^WT; n = 5 biological replicates [replicates 1–3 from Yuvaraj et al. (2021)]; n_total = 15). Asterisks (*) indicate significantly higher responses in ItypOrco^HsCO as compared to ItypOrco^WT (p = 0.002 to <0.001). (B) Cells transfected with either the wildtype or codon optimized Orco gene from D. ponderosae (DponOrco^WT and DponOrco^HsCO) failed to respond to VUAA1 (n = 3 biological replicates, n_total = 9), with similar Δfluorescence values in induced and non-induced cells (not shown in graph for clarity; see Supplementary Data 1). Error bars show SEM.