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. 2021 Sep 6;12:735643. doi: 10.3389/fimmu.2021.735643

Figure 5.

Figure 5

Elevated level of IMA gene signature expression correlates with IFNG expression by DEJ CD8TRM cells in HSV-2 post-healing human skin. (A) Cell density (upper) and IFNG gene expression (lower) of DEJ CD8TRM in skin biopsies taken at the sites of 8-week post-healing and their matched contralateral controls (n = 8). DEJ CD8TRM cells were laser microdissected and coupled with quantitative RT-PCR. IFNG gene expression relative to ACTB. Individual (B) and collective levels (C) of IMA gene signature expression in the 8-week and control set of skin biopsies with or without IFNG detection. (D) Micrographic images depicting spatial expression of IFI16, CD8 and IFNG mRNA transcripts in skin epidermis using RNA FISH dual probe sets of IFI16 + IFNG or IFI16 + CD8 in adjacent tissue sections of an 8-week skin biopsy, and dual probe set for IFI16 + CD8 on matched normal control skin. Scale bar, 100 µm.