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. 2021 Sep 6;9:618492. doi: 10.3389/fcell.2021.618492

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Copyright © 2021 Lou, Zhou, Li, Lu, Bao, Yang, Liao, Chen, Fang, Yang, Lyu and Zheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ACC1-knockdown (KD) fibroblasts and the cell motility capacity measurement. (A) The ACC1 protein content of ACC1-KD fibroblast cell lines, and (B) the ACC1 enzyme activity. The wound healing assay was carried out at different time points (0, 12, and 24 h) (C) without or (E) with palmitate (scale bar = 30 μm), and (D,F) show quantitative analysis, respectively. The Trans-well migration capacity at 100× magnification (upper) and 200× magnification (lower) (G) with or (I) without palmitate, respectively. (H,J) The quantitative analyses, respectively. All data were from three independent replicates and analyzed by mean ± SEM. Independent t-test in panels (A,B,D,F,H,J). *p < 0.05, **p < 0.01, ***p < 0.001.