FIGURE 6.

Neuronal death induced by Aβ peptides was alleviated by EG. (A) PC12 cells treated with amyloid peptides Aβ₄₂ (upper) and Aβ₄₀ (lower) for 24 h, at gradient concentrations from 0.01 to 50 μM, followed by Cell Counting Kit-8 (CCK-8) assay for viability. (B) PC12 cells pre-treated with (gray bar) or without (white bar) EG at 10⁻⁷ M for 18 h, then further subjected to 20 μM final concentration of amyloid peptides Aβ₄₀ and Aβ₄₂ for 24 h followed by CCK-8 assay for viability. The mean values (±S.D.) of three independent experiments are shown. An asterisk indicates statistical significance (*p < 0.05, **p < 0.001, ***p<<0.001). EG: emodin-8-O-β-d-glucopyranoside; NC: negative control.