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. 2021 Sep 6;12:735876. doi: 10.3389/fphar.2021.735876

FIGURE 7.

FIGURE 7

Cell death promoting effect of Aβ42 in 5-HT1B over-expressed stable PC12 cells eliminated by TNF-α signaling reduction via 5-HT1B pathway activation. A. TNF-α mRNA level induced by Aβ42 (20 μM).5-HT1B agonists (CP-94253 and dihydroergotamine) and EG added as indicated at 10−7 M. To confirm 5-HT1B mediated pathway role, the 5-HT1B was blocked by its selective antagonist SB 224289 (10−7 M), and the TNF-α mRNA level was measured in the presence of dihydroergotamine, CP-94253 and EG. The mean values (±S.D.) of three independent experiments are shown. Asterisks indicate statistical significance (***p<<0.001). B. Compounds shown in A were separately tested for their effects on inducing TNF-α expression in 5-HT1B over-expressed, stable PC12 cells. C, D, E. 5-HT1B over-expressed stable PC12 cells were pre-treated with the CP-94253, dihydroergotamine, and EG at 10−7 M for 18h, then subjected to Aβ42 (20 μM) or not, for 24 h, followed by CCK-8, MTT Kit viability assay and LDH for cytotoxicity. SB 224289 was applied 20 min before CP-94253, dihydroergotamine, and EG when necessary.