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. 2021 May 24;239(4):856–868. doi: 10.1111/joa.13458

FIGURE 1.

FIGURE 1

Negative controls for the antibodies used in this study. (a): The primary antibodies (goat anti‐SOM and rabbit anti‐NPY) were omitted from their respective incubation baths, and sections were reacted with the respective biotinylated secondary antibodies and streptavidin before being counterstained with thionin. (b): Section incubated with primary antibody goat anti‐SOM followed by a biotinylated goat anti‐rabbit antibody and streptavidin. No immunoreaction was detected. See also controls carried out for the same antibodies in a previous work (Cebada‐Sánchez et al., 2014). Abbreviations: DG: dentate gyrus; CA1: ammonic hippocampal field 1; CA2: ammonic hippocampal field 2. Scale bar: 200 µm