FIGURE 4.

Inhibition of SIRT1 weakened the protective effect of 8-MOP on chondrocytes. On the basis of IL-1β stimulation, the chondrocytes were dealt with 8-MOP (40 μM) and/or EX527 (20 μM) for 24 h. (A–B), MTT and FCM were adopted to define cell proliferation and apoptosis, respectively. (C). The expression of Bax, Bcl2, and cleaved Caspase3 was checked by Western blot. (D–E). The levels of inflammatory cytokines (IL-6, TNF-α, and IL-18) were detected by qRT-PCR and ELISA. (F–G): The oxidative stress factors (MDA, SOD, and GSH-PX) in chondrocytes were measured by the MDA, SOD, and GSH-PX detection kits. *p < 0.05, **p < 0.01, ***p < 0.001. (n = 3).